Recombinant Histone H4 抗体 (meLys20) (Sortase,His tag,AVI tag)
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Quick Overview for Recombinant Histone H4 抗体 (meLys20) (Sortase,His tag,AVI tag) (ABIN6731025)
抗原
See all Histone H4 (HISTH4) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- meLys20
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原理
- AbFlex Histone H4K20me1 antibody (rAb)
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品牌
- AbFlex®
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产品特性
- AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.
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亚型
- IgG2a
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anti-Histone H4 (HISTH4) (acLys12) antibody
HISTH4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (2meLys20) antibodyHISTH4 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (3meLys20) antibodyHISTH4 适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys8) antibodyHISTH4 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys5) antibodyHISTH4 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone H4 (HISTH4) (H4K16ac) antibodyHISTH4 适用: 人, 小鼠, Saccharomyces cerevisiae, Drosophila melanogaster WB, IF, ChIP, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (AA 2-103) antibodyHISTH4 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡, 马, 豚鼠, 犬, Cat, 猕猴 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (meArg3) antibodyHISTH4 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys8) antibodyHISTH4 适用: 人, 小鼠 WB, IF, ICC, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (HISTH4) (acLys5) antibodyHISTH4 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB*: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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说明
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This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Histone H4 (HISTH4)
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别名
- Histone H4
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背景
- AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/ exit points.
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分子量
- 12 kDa
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NCBI登录号
- NP_778224
抗原
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