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Histone H4 抗体 (H4K16ac)

Name: Histone H4 抗体 (H4K16ac)
SKU: ABIN2668880
Availability: OutOfStock

Cited in 15+ publications. The 兔多克隆 anti-Histone H4 antibody (ABIN2668880) specifically detects Histone H4 in WB, IF, ChIP, DB, ChIP-seq 和 CUT&Tag. The antibody is reactive with 人, 小鼠, Saccharomyces cerevisiae 和 Drosophila melanogaster samples.

Histone H4K16ac antibody (pAb) tested by Western blot. (Histone H4 抗体 (H4K16ac))

Histone H4K16ac antibody (pAb) tested by dot blot analysis. (Histone H4 抗体 (H4K16ac))

Histone H4K16ac antibody (pAb) specificity tested by peptide array analysis. (Histone H4 抗体 (H4K16ac))

ChIP

ChIP-seq

ChIP

5 images

(15 references)

产品编号 ABIN2668880

发货至: 中国

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中国
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Room 801-803

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Quick Overview for Histone H4 抗体 (H4K16ac) (ABIN2668880)

Key Features

High quality Histone H4 Primary Antibody for the detection of HISTH4.
Reliable product with high standard validation data

抗原

See all Histone H4 (HISTH4) 抗体

Histone H4 (HISTH4)

适用

人, 小鼠, Saccharomyces cerevisiae, Drosophila melanogaster

宿主

兔

克隆类型

多克隆

标记

This Histone H4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Discover our top product HISTH4 Primary Antibody

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H4K16ac

原理

Histone H4K16ac antibody (pAb)

纯化方法

Unpurified

免疫原

This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

Product Specific Information

What can the Histone H4 Antibody ABIN2668880 be used for?

This polyclonal Histone H4 Antibody detects Histone H4. The Histone H4 Antibody has been validated for various applications and can be used for the detection of Histone H4 and derivatives by Western Blotting, Immunofluorescence, Chromatin Immunoprecipitation, Dot Blot, ChIP DNA-Sequencing, Cleavage Under Targets and Tagmentation.

What validation data is available for this Histone H4 Antibody?

The Primary Antibody is referenced in 15 publications and is characterized by a proven, very high reliability. It has currently 5 product images that show its performance in a variety of applications. Histone H4 Antibody for the detection of Histone H4 and derivatives.

What is the function of Histone H4?

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails\; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated\; this record represents the centromeric copy. [provided by RefSeq, Jul 2008].
anti-Histone H4 (HISTH4) (acLys12) antibody
HISTH4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (2meLys20) antibody
HISTH4 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (3meLys20) antibody
HISTH4 适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (acLys8) antibody
HISTH4 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (acLys5) antibody
HISTH4 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Histone H4 (HISTH4) (AA 2-103) antibody
HISTH4 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡, 马, 豚鼠, 犬, Cat, 猕猴 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (meArg3) antibody
HISTH4 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (acLys5) antibody
HISTH4 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (acLys8) antibody
HISTH4 适用: 人, 小鼠 WB, IF, ICC, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H4 (HISTH4) (acetylated) antibody
HISTH4 适用: 人, 小鼠 WB, IHC, IF, ICC, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:1,000 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. Individual optimization may be required.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Verrier, Taglini, Barrales, Webb, Urano, Braun, Bayne: "Global regulation of heterochromatin spreading by Leo1." in: Open biology, Vol. 5, Issue 5, (2015) (PubMed).

Sedic, Skibinski, Brown, Gallardo, Mulligan, Martinez, Keller, Glover, Richardson, Cowan, Toland, Ravichandran, Riethman, Naber, Näär, Blasco, Hinds, Kuperwasser: "Haploinsufficiency for BRCA1 leads to cell-type-specific genomic instability and premature senescence." in: Nature communications, Vol. 6, pp. 7505, (2015) (PubMed).

Korb, Herre, Zucker-Scharff, Darnell, Allis: "BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice." in: Nature neuroscience, Vol. 18, Issue 10, pp. 1464-73, (2015) (PubMed).

Froyd, Kapoor, Dietrich, Rusche: "The deacetylase Sir2 from the yeast Clavispora lusitaniae lacks the evolutionarily conserved capacity to generate subtelomeric heterochromatin." in: PLoS genetics, Vol. 9, Issue 10, pp. e1003935, (2013) (PubMed).

Wang, Tadeo, Hou, Tu, Thompson, Yates, Jia: "Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries." in: Genes & development, Vol. 27, Issue 17, pp. 1886-902, (2013) (PubMed).

Thijssen, Tobi, Balog, Schouten, Kremer, El Bouazzaoui, Henneman, Putter, Eline Slagboom, Heijmans, van der Maarel: "Chromatin remodeling of human subtelomeres and TERRA promoters upon cellular senescence: commonalities and differences between chromosomes." in: Epigenetics, Vol. 8, Issue 5, pp. 512-21, (2013) (PubMed).

Badeaux, Yang, Cardenas, Vemulapalli, Chen, Kusewitt, Richie, Li, Bedford: "Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 429-37, (2012) (PubMed).

Krogan, Hogan, Long: "APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19." in: Development (Cambridge, England), Vol. 139, Issue 22, pp. 4180-90, (2012) (PubMed).

Yuan, Rossetto, Mellert, Dang, Srinivasan, Johnson, Hodawadekar, Ding, Speicher, Abshiru, Perry, Wu, Yang, Zheng, Speicher, Thibault, Verreault, Johnson, Berger, Sternglanz, McMahon, Côté, Marmorstein: "MYST protein acetyltransferase activity requires active site lysine autoacetylation." in: The EMBO journal, Vol. 31, Issue 1, pp. 58-70, (2012) (PubMed).

Larsson, Ulfhammer, Magnusson, Bergh, Lunke, El-Osta, Medcalf, Svensson, Karlsson, Jern: "Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression." in: PLoS ONE, Vol. 7, Issue 2, pp. e31573, (2012) (PubMed).

McCullough, Xu, Dent, Bekiranov, Roeder, Grant: "Reelin is a target of polyglutamine expanded ataxin-7 in human spinocerebellar ataxia type 7 (SCA7) astrocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 52, pp. 21319-24, (2012) (PubMed).

Liu, McConnell, Dixon, Calvi: "Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex." in: Molecular biology of the cell, Vol. 23, Issue 1, pp. 200-12, (2012) (PubMed).

Sotillo, Laver, Mellert, Schelter, Cleary, McMahon, Thomas-Tikhonenko: "Myc overexpression brings out unexpected antiapoptotic effects of miR-34a." in: Oncogene, Vol. 30, Issue 22, pp. 2587-94, (2011) (PubMed).

Regnard, Straub, Mitterweger, Dahlsveen, Fabian, Becker: "Global analysis of the relationship between JIL-1 kinase and transcription." in: PLoS genetics, Vol. 7, Issue 3, pp. e1001327, (2011) (PubMed).

Kumari, Biacsi, Usdin: "Repeat expansion affects both transcription initiation and elongation in friedreich ataxia cells." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4209-15, (2011) (PubMed).
抗原

Histone H4 (HISTH4)

别名

Histone H4

背景

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SMARCA5 (SNF2h)-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4K16ac) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA.

分子量

8 kDa

基因ID

121504

NCBI登录号

NP_778224