Histone H3.3 抗体 (K27M)
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北京 101111
Quick Overview for Histone H3.3 抗体 (K27M) (ABIN6731017)
抗原
See all Histone H3.3 (H3F3A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- K27M
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原理
- Histone H3.3K27M antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This antibody was generated using a Peptide containing a Methionine substitution at lysine 27 of human Histone H3.
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亚型
- IgG
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anti-H3 Histone, Family 3A (H3F3A) (pSer31) antibody
H3F3A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (AA 2-136) antibodyH3F3A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (AA 1-137) antibodyH3F3A 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2D7-H1 unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, ICC, FACS, IF (p) 宿主: 兔 Monoclonal 23GB6095 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) (H3S31ph) antibodyKD Validated H3F3A 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 25GB2465 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (H3.3T3ph) antibodyH3F3A 适用: 人 WB, ELISA, ICC, FACS 宿主: 兔 Monoclonal 28H4 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 ELISA, IHC, IF 宿主: 兔 Monoclonal 4H2 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, IHC (p) 宿主: 兔 Monoclonal 7B12 unconjugated
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应用备注
- Western Blot: 1:500 - 1:1,000 dilution
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- Histone H3
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背景
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription.Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78 % of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36 % of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.
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NCBI登录号
- NP_002098
抗原
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