DYKDDDDK Tag 抗体 (DyLight 405)
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北京 101111
Quick Overview for DYKDDDDK Tag 抗体 (DyLight 405) (ABIN6720680)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-346-383
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Supplier
- Rockland
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原理
- DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 405 Conjugated
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交叉反应 （详细）
- The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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纯化方法
- This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.
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免疫原
- This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
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亚型
- IgG2a kappa
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Labeling Ratio
- 3.4
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (Agarose Beads)WB, IP 宿主: 小鼠 Monoclonal 29E4-G7 Agarose Beads
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应用备注
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FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:10,000 - 1:25,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000-1:25,000
Other: User Optimized
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说明
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Anti-FLAG DyLight™405 has been tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DYKDDDDK Tag
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背景
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Synonyms: mouse anti-FLAG™ tag DyLight™405 conjugation, Enterokinase Cleavage Site (ECS), DyLight™405 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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