Biotin 抗体 (DyLight 405)
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北京 101111
Quick Overview for Biotin 抗体 (DyLight 405) (ABIN6720658)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-446-098
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Supplier
- Rockland
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原理
- Biotin Antibody Dylight™ 405 Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
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纯化方法
- Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
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免疫原
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
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亚型
- IgG
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Labeling Ratio
- 3.0
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anti-Biotin antibody
WB, IHC, ELISA, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyWB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
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应用备注
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FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000-1:25,000
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说明
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The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-Biotin antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: rabbit anti-Biotin Antibody Dylight™ 405 Conjugated, rabbit anti Biotin Antibody Dylight™ 405 Conjugated
Background: Anti-Biotin antibody conjugated to DyLight 405 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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