CRB1 抗体
Quick Overview for CRB1 抗体 (ABIN6719658)
抗原
See all CRB1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-CRB1 Antibody Picoband®
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序列
- FRTRDANVII LHAEKEPEFL NISIQDSRLF FQLQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CRB1 Antibody Picoband® (ABIN6719658). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human CRB1, which shares 87.9% and 84.8% amino acid (aa) sequence identity with mouse and rat CRB1, respectively.
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亚型
- IgG
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anti-Crumbs Homolog 1 (CRB1) (AA 20-200) antibody
CRB1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Crumbs Homolog 1 (CRB1) (AA 26-134) antibodyCRB1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Crumbs Homolog 1 (CRB1) (AA 20-200) antibodyCRB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: CRB1 crumbs homolog 1 (Drosophila)". 2. den Hollander, A. I., Davis, J., van der Velde-Visser, S. D., Zonneveld, M. N., Pierrottet, C. O., Koenekoop, R. K., Kellner, U., van den Born, L. I., Heckenlively, J. R., Hoyng, C. B., Handford, P. A., Roepman, R., Cremers, F. P. M. CRB1 mutation spectrum in inherited retinal dystrophies. Hum. Mutat. 24: 355-369, 2004. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CRB1 (Crumbs Homolog 1 (CRB1))
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别名
- CRB1
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背景
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Synonyms: Protein crumbs homolog 1, CRB1
Tissue Specificity: Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye.
Background: Crumbs homolog 1 is a protein that in humans is encoded by the CRB1 gene. This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.
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分子量
- 154 kDa
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基因ID
- 23418
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UniProt
- P82279
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途径
- Notch Signaling
抗原
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