AKR1D1 抗体
Quick Overview for AKR1D1 抗体 (ABIN6719640)
抗原
See all AKR1D1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-AKR1D1 Antibody Picoband®
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序列
- EEMKDIEALN KNVRFVELLM WRDHPEYPFH DEY
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AKR1D1 Antibody Picoband® (ABIN6719640). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1D1, which shares 90.9% and 93.9% amino acid (aa) sequence identity with mouse and rat AKR1D1, respectively.
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亚型
- IgG
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anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 227-326) antibody
AKR1D1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-200) antibodyAKR1D1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 227-326) antibodyAKR1D1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) antibodyAKR1D1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) antibodyAKR1D1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 113-162) antibodyAKR1D1 适用: 人, 大鼠, Cow, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) antibodyAKR1D1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
1. Charbonneau, A., Luu-The, V. Assignment of steroid 5-beta-reductase (SRD5B1) and its pseudogene (SRD5BP1) to human chromosome bands 7q32-q33 and 1q23-q25, respectively, by in situ hybridization. Cytogenet. Cell Genet. 84: 105-106, 1999. 2. Charbonneau, A., The, V.-L. Genomic organization of a human 5-beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme. Biochim. Biophys. Acta 1517: 228-235, 2001. 3. Clayton, P. T., Mills, K. A., Johnson, A. W., Barabino, A., Marazzi, M. G. Delta 4-3-oxosteroid 5 beta-reductase deficiency: failure of ursodeoxycholic acid treatment and response to chenodeoxycholic acid plus cholic acid. Gut 38: 623-628, 1996. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
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别名
- AKR1D1
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背景
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Synonyms: 3-oxo-5-beta-steroid 4-dehydrogenase, Aldo-keto reductase family 1 member D1, Delta (4)-3-ketosteroid 5-beta-reductase, Delta (4)-3-oxosteroid 5-beta-reductase, AKR1D1, SRD5B1
Tissue Specificity: Highly expressed in liver. Expressed in testis and weakly in colon.
Background: Human delta (4)-3-oxosteroid 5-beta-reductase (steroid 5-beta-reductase) catalyzes 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta (4)-3-one structure. This gene is mapped to 7q33. The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta (4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.
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分子量
- 37 kDa
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基因ID
- 6718
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UniProt
- P51857
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途径
- Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
抗原
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