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NONO 抗体 (N-Term)

This 小鼠 单克隆 anti-NONO antibody (Clone 11E2) specifically detects NONO in WB, IF, ICC 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN6719600
发货至: 中国
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Quick Overview for NONO 抗体 (N-Term) (ABIN6719600)

抗原

See all NONO 抗体
NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

适用

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宿主

  • 81
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小鼠

克隆类型

  • 62
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单克隆

标记

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This NONO antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

11E2
  • 抗原表位

    • 15
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    AA 1-35, N-Term

    原理

    Anti-nmt55 p54nrb NONO Antibody (monoclonal, 11E2)

    序列

    MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQ

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-nmt55 p54nrb NONO Antibody (monoclonal, 11E2) (ABIN6719600). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    免疫原

    A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.

    亚型

    IgG1
  • 应用备注

    Western blot, 0.1-0.5 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 5 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells

    说明

    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

    别名

    NONO

    背景

    Synonyms: Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, p54 (nrb), p54nrb, NONO, NRB54

    Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

    Background: Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

    分子量

    60 kDa

    基因ID

    4841

    UniProt

    Q15233
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