GLI2 抗体 (AA 721-1457)
Quick Overview for GLI2 抗体 (AA 721-1457) (ABIN6719569)
抗原
See all GLI2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 721-1457
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原理
- Anti-Zinc finger protein GLI2 GLI2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Zinc finger protein GLI2 GLI2 Antibody (ABIN6719569). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human GLI2 recombinant protein (Position: K721-D1457).
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亚型
- IgG
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anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody
GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1287-1321), (C-Term) antibodyGLI2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB49147 unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (C-Term) antibodyGLI2 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 600-649) antibodyGLI2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 30-65) antibodyGLI2 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibodyGLI2 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 2-20) antibodyGLI2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (N-Term) antibodyGLI2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1444-1463), (C-Term) antibodyGLI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bertolacini, C. D. P., Ribeiro-Bicudo, L. A., Petrin, A., Richieri-Costa, A., Murray, J. C. Clinical findings in patients with GLI2 mutations--phenotypic variability. Clin. Genet. 81: 70-75, 2012. 2. Matsumoto, N., Fujimoto, M., Kato, R., Niikawa, N. Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization. Genomics 36: 220-221, 1996. 3. Roessler, E., Ermilov, A. N., Grange, D. K., Wang, A., Grachtchouk, M., Dlugosz, A. A., Muenke, M. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Molec. Genet. 14: 2181-2188, 2005. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- GLI2 (GLI Family Zinc Finger 2 (GLI2))
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别名
- GLI2
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背景
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Synonyms: Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein, GLI2, THP
Tissue Specificity: Expressed in breast cancers. Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines. Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines.
Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.
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分子量
- 210 kDa
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基因ID
- 2736
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UniProt
- P10070
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis
抗原
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