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Caspase 3 抗体 (AA 67-175)

Name: Caspase 3 抗体 (AA 67-175)
SKU: ABIN6719555
Availability: OutOfStock

The 小鼠单克隆 anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from 人. It has been validated for WB, IHC, ICC, FACS 和 IF.

Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (ABIN6719555). (Caspase 3 抗体 (AA 67-175))

IF analysis of Caspase-3 using anti-Caspase-3 antibody (ABIN6719555). (Caspase 3 抗体 (AA 67-175))

IHC

IHC analysis of Caspase-3 using anti-Caspase-3 antibody (ABIN6719555). (Caspase 3 抗体 (AA 67-175))

IHC

Flow Cytometry analysis of HepG2 cells using anti- Caspase-3 antibody (ABIN6719555). (Caspase 3 抗体 (AA 67-175))

FACS

5 images

产品编号 ABIN6719555

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Caspase 3 抗体 (AA 67-175) (ABIN6719555)

抗原

See all Caspase 3 (CASP3) 抗体

Caspase 3 (CASP3)

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Caspase 3 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

15G8

Discover our top product CASP3 Primary Antibody

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AA 67-175

原理

Anti-Caspase-3 Antibody (monoclonal, 15G8)

特异性

No cross reactivity with other proteins.

交叉反应（详细）

No cross-reactivity with other proteins.

预测反应

Human Caspase-3 shares 86%,90% amino acid (aa) sequence identity with mouse,rat Caspase-3,respectively.

产品特性

Anti-Caspase-3 Antibody.(monoclonal, 15G8)-7. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human Caspase-3 recombinant protein (Position: T67-D175). Human Caspase-3 shares 86% and 90% amino acid (aa) sequence identity with mouse and rat Caspase-3, respectively.

亚型

IgG1
anti-Caspase 3 (CASP3) (AA 60-100) antibody
CASP3 适用: 人, 小鼠, 大鼠, Cow, 山羊 WB, IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 67-175) antibody
CASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) (C-Term) antibody
CASP3 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 11-120) antibody
CASP3 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) antibody
CASP3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal 5E1 unconjugated

anti-Caspase 3 (CASP3) (cleaved) antibody
CASP3 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 29-175) antibody
CASP3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Caspase 3 (CASP3) (AA 29-175) antibody
CASP3 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Caspase 3 (CASP3) (AA 60-90) antibody
CASP3 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14696 unconjugated

anti-Caspase 3 (CASP3) (AA 149-179), (Asp175) antibody
CASP3 适用: 人, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB25834 unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

说明

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
抗原

Caspase 3 (CASP3)

别名

CASP3

背景

Background: Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
Gene Full Name: caspase 3

分子量

20 kDa

基因ID

836

UniProt

P42574

途径

Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation