ENO2/NSE 抗体
Quick Overview for ENO2/NSE 抗体 (ABIN6719549)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-NSE/ENO2 Antibody Picoband®
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序列
- LKAVDHINST IAPALISSGL SVVEQEKLDN LMLELDGTEN K
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NSE/ENO2 Antibody Picoband® (ABIN6719549). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human NSE, which shares 95.1% and 100% amino acid (aa) sequence identity with mouse and rat NSE, respectively.
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亚型
- IgG
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibody
ENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) antibodyENO2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Craig, S. P., Day, I. N. M., Thompson, R. J., Craig, I. W. : Localization of human neurone-specific enolase to chromosome 12p13. (Abstract) Cytogenet. Cell Genet. 51: 980 only, 1989. 2. Craig, S. P., Day, I. N. M., Thompson, R. J., Craig, I. W. : Localisation of neurone-specific enolase (ENO2) to 12p13. Cytogenet. Cell Genet. 54: 71-73, 1990. 3. Mattei, J. F., Baeteman, M. A., Mattei, M. G., Ardissonne, J. P., Giraud, F. : Regional assignments of CS and ENO2 on chromosome 12. (Abstract) Cytogenet. Cell Genet. 32: 297 only, 1982. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- ENO2
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背景
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Synonyms: Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Tissue Specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Background: NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
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分子量
- 47 kDa
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基因ID
- 2026
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UniProt
- P09104
抗原
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