RNF169 抗体 (AA 311-708)
Quick Overview for RNF169 抗体 (AA 311-708) (ABIN6719530)
抗原
See all RNF169 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 311-708
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原理
- Anti-RNF169 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-R Antibody Picoband® (ABIN6719530). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RNF169 recombinant protein (Position: N311-K708).
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亚型
- IgG
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anti-Ring Finger Protein 169 (RNF169) antibody
RNF169 适用: 人, 大鼠, 小鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 169 (RNF169) (AA 643-742) antibodyRNF169 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 169 (RNF169) (AA 643-742) antibodyRNF169 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Ring Finger Protein 169 (RNF169) (AA 215-264) antibodyRNF169 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Bat, 小鸡, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 169 (RNF169) (N-Term) antibodyRNF169 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Chen J, Feng W, Jiang J, Deng Y, Huen MS. Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage. J Biol Chem. 2012 Aug 10,287(33):27715-22. doi: 10.1074/jbc.M112.373530. Epub 2012 Jun 25. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RNF169 (Ring Finger Protein 169 (RNF169))
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别名
- RNF169
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背景
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Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991
Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Background: RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair. This gene is mapped to 11q13.4.
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分子量
- 90 kDa
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基因ID
- 254225
抗原
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