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Galectin 3 抗体 (AA 139-250)

The 小鼠 单克隆 anti-Galectin 3 antibody is suitable to detect Galectin 3 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC, ICC, IF 和 FACS.
产品编号 ABIN6719486
发货至: 中国
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Quick Overview for Galectin 3 抗体 (AA 139-250) (ABIN6719486)

抗原

See all Galectin 3 (LGALS3) 抗体
Galectin 3 (LGALS3)

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Galectin 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

12B12
  • 抗原表位

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    AA 139-250

    原理

    Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12) (ABIN6719486). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.

    亚型

    IgG2a
  • 应用备注

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 2 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells

    说明

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    Galectin 3 (LGALS3)

    别名

    LGALS3

    背景

    Synonyms: Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2

    Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

    Background: SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

    分子量

    28 kDa

    基因ID

    3958

    UniProt

    P17931

    途径

    RTK signaling
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