SFRP1 抗体
Quick Overview for SFRP1 抗体 (ABIN6719484)
抗原
See all SFRP1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-SFRP1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SFRP1 Antibody Picoband® (ABIN6719484). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human SFRP1 recombinant protein (A204-K314).
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亚型
- IgG
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anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 8-153) antibody
SFRP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 201-314) antibodySFRP1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 170-240) antibodySFRP1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 26-55), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB21557 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) antibodySFRP1 适用: 人 WB, IHC, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 30-314) antibodySFRP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 33-63), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Baylin, S. B., Herman, J. G. DNA hypermethylation in tumorigenesis: epigenetics joins genetics. Trends Genet. 16: 168-174, 2000. 2. Cameron, E. E., Bachman, K. E., Myohanen, S., Herman, J. G., Baylin, S. B. Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nature Genet. 21: 103-107, 1999. 3. Finch, P. W., He, X., Kelley, M. J., Uren, A., Schaudies, R. P., Popescu, N. C., Rudikoff, S., Aaronson, S. A., Varmus, H. E., Rubin, J. S. Purification and molecular cloning of a secreted, frizzled-related antagonist of Wnt action. Proc. Nat. Acad. Sci. 94: 6770-6775, 1997. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
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别名
- SFRP1
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背景
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Synonyms: Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2, SFRP1, FRP, FRP1, SARP2
Tissue Specificity: Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
Background: Secreted frizzled-related protein 1, also known as SFRP1, is a protein which in humans is encoded by the SFRP1 gene. It is mapped to 8p11.21. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling, epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.
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分子量
- 35 kDa
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基因ID
- 6422
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UniProt
- Q8N474
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
抗原
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