DOK7 抗体
Quick Overview for DOK7 抗体 (ABIN6719428)
抗原
See all DOK7 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-DOK7 Antibody Picoband®
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序列
- STVEERVAQE ALETLQLEKR LSLLSHAGR
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DOK7 Antibody Picoband® (ABIN6719428). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human DOK7, which shares 86.2% amino acid (aa) sequence identity with mouse DOK7.
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亚型
- IgG
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anti-Docking Protein 7 (DOK7) antibody
DOK7 适用: 人 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 7E2 unconjugated
anti-Docking Protein 7 (DOK7) (N-Term) antibodyDOK7 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) (AA 416-504) antibodyDOK7 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) (C-Term) antibodyDOK7 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) antibodyDOK7 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C6 unconjugated
anti-Docking Protein 7 (DOK7) antibodyDOK7 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 3C12 unconjugated
anti-Docking Protein 7 (DOK7) (AA 63-320) antibodyDOK7 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) (AA 10-59) antibodyDOK7 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) (AA 21-120) antibodyDOK7 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Docking Protein 7 (DOK7) (AA 402-451) antibodyDOK7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
1. Arimura, S., Okada, T., Tezuka, T., Chiyo, T., Kasahara, Y., Yoshimura, T., Motomura, M., Yoshida, N., Beeson, D., Takeda, S., Yamanashi, Y. DOK7 gene therapy benefits mouse models of diseases characterized by defects in the neuromuscular junction. Science 345: 1505-1508, 2014. 2. Beeson, D., Higuchi, O., Palace, J., Cossins, J., Spearman, H., Maxwell, S., Newsom-Davis, J., Burke, G., Fawcett, P., Motomura, M., Muller, J. S., Lochmuller, H., Slater, C., Vincent, A., Yamanashi, Y. Dok-7 mutations underlie a neuromuscular junction synaptopathy. Science 313: 1975-1978, 2006. 3. Hallock, P. T., Xu, C.-F., Park, T.-J., Neubert, T. A., Curran, T., Burden, S. J. Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L. Genes Dev. 24: 2451-2461, 2010. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DOK7 (Docking Protein 7 (DOK7))
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别名
- DOK7
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背景
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Synonyms: Protein Dok-7, Downstream of tyrosine kinase 7, DOK7, C4orf25,
Tissue Specificity: Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .
Background: Dok-7 is a non-catalytic cytoplasmic adaptor protein that is expressed specifically in muscle and is essential for the formation of neuromuscular synapses. Further, Dok-7 contains pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains that are critical for Dok-7 function. It is mapped to 4p16.3. The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants.
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分子量
- 65 kDa
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基因ID
- 285489
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途径
- Skeletal Muscle Fiber Development
抗原
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