Dihydrofolate Reductase 抗体 (AA 2-187)
Quick Overview for Dihydrofolate Reductase 抗体 (AA 2-187) (ABIN6719418)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2-187
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原理
- Anti-DHFR Antibody Picoband® (monoclonal, 3C8)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DHFR Antibody Picoband® (monoclonal, 3C8) (ABIN6719418). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.
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亚型
- IgG2b
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anti-Dihydrofolate Reductase (DHFR) (AA 88-187) antibody
DHFR 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6G7 unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 2-187) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Monoclonal 9B2 unconjugated Recombinant Antibody
anti-Dihydrofolate Reductase (DHFR) (AA 135-164) antibodyDHFR 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-50) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 16-30), (N-Term) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49346 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-187) antibodyDHFR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, IHC (p), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
1.Chen MJ, Shimada T, Moulton AD, Harrison M, Nienhuis AW (December 1982). "Intronless human dihydrofolate reductase genes are derived from processed RNA molecules". Proc. Natl. Acad. Sci. U.S.A. 79 (23): 7435-9. 2.Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW (March 1984). "The functional human dihydrofolate reductase gene". J. Biol. Chem. 259 (6): 3933-43. 3.Funanage VL, Myoda TT, Moses PA, Cowell HR (October 1984). "Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5". Mol. Cell. Biol. 4 (10): 2010-6. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Dihydrofolate Reductase (DHFR)
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别名
- DHFR
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背景
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Synonyms: Dihydrofolate reductase, DHFR
Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Background: SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
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分子量
- 22 kDa
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基因ID
- 1719
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UniProt
- P00374
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途径
- Mitotic G1-G1/S Phases
抗原
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