ACLY 抗体 (AA 1-180)
Quick Overview for ACLY 抗体 (AA 1-180) (ABIN6719406)
抗原
See all ACLY 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-180
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原理
- Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2) (ABIN6719406). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.
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亚型
- IgG2b
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anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody
ACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5F8D11 unconjugated
anti-ATP Citrate Lyase (ACLY) (AA 752-1101) antibodyACLY 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATP Citrate Lyase (ACLY) (pSer455) antibodyACLY 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibodyACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-ATP Citrate Lyase (ACLY) (AA 4-265) antibodyACLY 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATP Citrate Lyase (ACLY) antibodyACLY 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-ATP Citrate Lyase (ACLY) antibodyACLY 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal 3A5 unconjugated Recombinant Antibody
anti-ATP Citrate Lyase (ACLY) (C-Term) antibodyACLY 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-ATP Citrate Lyase (ACLY) antibodyKD Validated ACLY 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1620 unconjugated Recombinant Antibody
anti-ATP Citrate Lyase (ACLY) (Middle Region) antibodyACLY 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 斑马鱼, 绵羊, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
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说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ACLY (ATP Citrate Lyase (ACLY))
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别名
- ACLY
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背景
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Synonyms: ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY
Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.
Background: ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
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分子量
- 121 kDa
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基因ID
- 47
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UniProt
- P53396
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途径
- Warburg Effect
抗原
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