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SP1 抗体 (AA 384-603)

This 兔 多克隆 anti-SP1 antibody specifically detects SP1 in WB, IHC, ELISA, IF, ICC 和 FACS. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN6719376
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SP1 抗体 (AA 384-603) (ABIN6719376)

抗原

See all SP1 抗体
SP1 (Sp1 Transcription Factor (SP1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This SP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
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    AA 384-603

    原理

    Anti-SP1 Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-SP1 Antibody (ABIN6719376). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived human SP1 recombinant protein (Position: Q384-A603).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
    Immunocytochemistry/Immunofluorescence, 5 μg/mL
    Immunofluorescence, 5 μg/mL
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL

    说明

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    SP1 (Sp1 Transcription Factor (SP1))

    别名

    SP1

    背景

    Synonyms: Transcription factor Sp1, SP1, TSFP1

    Tissue Specificity: Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.

    Background: Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

    分子量

    90 kDa

    UniProt

    P08047

    途径

    Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
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