EWSR1 抗体 (Middle Region)
Quick Overview for EWSR1 抗体 (Middle Region) (ABIN6719313)
抗原
See all EWSR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 369-399, Middle Region
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原理
- Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4)
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序列
- NDSVTLDDLA DFFKQCGVVK MNKRTGQPMI H
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) (ABIN6719313). Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human EWSR1, different from the related mouse sequence by one amino acid.
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亚型
- IgG2b
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anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (Internal Region) antibody
Verified EWSR1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (AA 358-453) antibodyEWSR1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5C10 unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (Middle Region) antibodyEWSR1 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) antibodyEWSR1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (Internal Region) antibodyEWSR1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (AA 358-453) antibodyEWSR1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A9 unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (AA 369-399), (Middle Region) antibodyEWSR1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (N-Term) antibodyEWSR1 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (AA 1-300) antibodyEWSR1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ewing Sarcoma Breakpoint Region 1 (EWSR1) (AA 358-453) antibodyEWSR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
1. "Entrez Gene: EWSR1 Ewing sarcoma breakpoint region 1". 2. Delattre O, Zucman J, Plougastel B, Desmaze C, Melot T, Peter M, Kovar H, Joubert I, de Jong P, Rouleau G (October 1992). "Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours". Nature 359 (6391): 162-5. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))
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别名
- EWSR1
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背景
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Synonyms: RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein, EWSR1, EWS
Tissue Specificity: Ubiquitous.
Background: This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11,22) (q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
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分子量
- 95 kDa
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基因ID
- 2130
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UniProt
- Q01844
抗原
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