Cortactin 抗体 (AA 1-105)
Quick Overview for Cortactin 抗体 (AA 1-105) (ABIN6719306)
抗原
See all Cortactin (CTTN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-105
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原理
- Anti-Cortactin/CTTN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Cortactin/CTTN Antibody Picoband® (ABIN6719306). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Cortactin recombinant protein (Position: M1-Q105).
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亚型
- IgG
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anti-Cortactin (CTTN) (N-Term) antibody
KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 214-513) antibodyCTTN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Cortactin (CTTN) (pTyr466) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 341-450) antibodyCTTN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (pTyr421) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (N-Term) antibodyVerified CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Tegtmeyer, N., Wittelsberger, R., Hartig, R., Wessler, S., Martinez-Quiles, N., Backert, S. Serine phosphorylation of cortactin controls focal adhesion kinase activity and cell scattering induced by Helicobacter pylori. Cell Host Microbe 9: 520-531, 2011. 2. Williams, M. R., Markey, J. C., Doczi, M. A., Morielli, A. D. An essential role for cortactin in the modulation of the potassium channel Kv1.2. Proc. Nat. Acad. Sci. 104: 17412-17417, 2007. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Cortactin (CTTN)
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别名
- CTTN
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背景
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Synonyms: Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Background: Cortactin, mapped to 11q13.3, is a multidomain protein. This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.
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分子量
- 85 kDa
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UniProt
- Q14247
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途径
- MAPK Pathway
抗原
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