CST3 抗体 (AA 31-146)
Quick Overview for CST3 抗体 (AA 31-146) (ABIN6719299)
抗原
See all CST3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 31-146
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原理
- Anti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) (ABIN6719299). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Cystatin C recombinant protein (Position: K31-A146).
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亚型
- IgG2a
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anti-Cystatin C (CST3) antibody
CST3 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Cystatin C (CST3) antibodyCST3 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cystatin C (CST3) antibodyCST3 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Cystatin C (CST3) antibodyCST3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5H2 unconjugated
anti-Cystatin C (CST3) antibodyCST3 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 3A1B7 unconjugated
anti-Cystatin C (CST3) (C-Term) antibodyVerified CST3 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Cystatin C (CST3) (Internal Region) antibodyVerified CST3 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Cystatin C (CST3) (Internal Region) antibodyCST3 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cystatin C (CST3) (AA 1-146) antibodyCST3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cystatin C (CST3) (AA 28-127) antibodyCST3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3D10 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA (Cap), 1-5 μg/mL
1. Abrahamson, M., Grubb, A. Increased body temperature accelerates aggregation of the leu68-to-gln mutant cystatin C, the amyloid-forming protein in hereditary cystatin C amyloid angiopathy. Proc. Nat. Acad. Sci. 91: 1416-1420, 1994. 2. Crawford, F. C., Freeman, M. J., Schinka, J. A., Abdullah, L. I., Gold, M., Hartman, R., Krivian, K., Morris, M. D., Richards, D., Duara, R., Anand, R., Mullan, M. J. A polymorphism in the cystatin C gene is a novel risk factor for late-onset Alzheimer's disease. Neurology 55: 763-768, 2000. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CST3 (Cystatin C (CST3))
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别名
- CST3
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背景
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Synonyms: Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439
Tissue Specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
Background: Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.
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分子量
- 15 kDa
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基因ID
- 1471
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UniProt
- P01034
抗原
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