LY9 抗体
Quick Overview for LY9 抗体 (ABIN6719290)
抗原
See all LY9 (CD229) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
 - Anti-CD229/LY9 Antibody
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序列
 - YKAQINQRNF EVTTEEEFTL FVYEQLQEPQ VTMK
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-/LY9 Antibody Picoband® (ABIN6719290). Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - A synthetic peptide corresponding to a sequence at the N-terminus of human CD229/LY9, which shares 55.9% and 61.8% amino acid (aa) sequence identity with mouse and rat CD229/LY9, respectively.
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亚型
 - IgG
 
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应用备注
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                        Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Sandrin MS, Henning MM, Lo MF, Baker E, Sutherland GR, McKenzie IF (Feb 1996). "Isolation and characterization of cDNA clones for Humly9: the human homologue of mouse Ly9". Immunogenetics. 2. Kingsmore SF, Souryal CA, Watson ML, Patel DD, Seldin MF (Aug 1995). "Physical and genetic linkage of the genes encoding Ly-9 and CD48 on mouse and human chromosomes 1". Immunogenetics. 3. "Entrez Gene: LY9 lymphocyte antigen 9". - 
                                            
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. 
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- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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别名
 - LY9
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背景
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Synonyms: T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229, LY9, CDABP0070
Tissue Specificity: Increased surface expression on T-cells of systemic lupus erythematosus (SLE) patients.
Background: T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229 (cluster of differentiation 229). LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP.
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分子量
 - 28 kDa
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基因ID
 - 4063
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途径
 - BCR Signaling
 
抗原
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