DARS2 抗体 (AA 334-448)
Quick Overview for DARS2 抗体 (AA 334-448) (ABIN6719279)
抗原
See all DARS2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 334-448
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原理
- Anti-DARS2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DARS2 Antibody Picoband® (ABIN6719279). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DARS2 recombinant protein (Position: D334-A448).
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亚型
- IgG
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anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) (AA 406-645) antibody
DARS2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) (AA 1-645) antibodyDARS2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) antibodyDARS2 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) (AA 1-645) antibodyDARS2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) (AA 489-628) antibodyDARS2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2) (AA 390-653) antibodyDARS2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells ELISA, 0.1-0.5 μg/mL
1. Isohanni, P., Linnankivi, T., Buzkova, J., Lonnqvist, T., Pihko, H., Valanne, L., Tienari, P. J., Elovaara, I., Pirttila, T., Reunanen, M., Koivisto, K., Marjavaara, S., Suomalainen, A. DARS2 mutations in mitochondrial leucoencephalopathy and multiple sclerosis. J. Med. Genet. 47: 66-70, 2010. 2. Miyake, N., Yamashita, S., Kurosawa, K., Miyatake, S., Tsurusaki, Y., Doi, H., Saitsu, H., Matsumoto, N. A novel homozygous mutation of DARS2 may cause a severe LBSL variant. (Letter) Clin. Genet. 80: 293-296, 2011. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))
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别名
- DARS2
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背景
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Synonyms: Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, DARS2
Tissue Specificity: Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Background: DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.
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分子量
- 74 kDa
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基因ID
- 55157
抗原
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