Amphiregulin 抗体 (AA 100-191)
Quick Overview for Amphiregulin 抗体 (AA 100-191) (ABIN6719275)
抗原
See all Amphiregulin (AREG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-191
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原理
- Anti-Amphiregulin/Areg Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Amphiregulin/Areg Antibody (ABIN6719275). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived mouse Amphiregulin recombinant protein (Position:V100-K191).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Rat
ELISA (Cap), 1-5 μg/mL, -
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说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Amphiregulin (AREG)
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别名
- Areg
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背景
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Synonyms: Amphiregulin, AR, Schwannoma-derived growth factor, SDGF, Areg, Sdgf
Tissue Specificity: Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor (EGF) family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. It is mapped to 9q32. It has been shown to play a role in immunity, inflammation, tissue repair, and lung and mammary gland development. Homozygous knockout mice for this gene exhibit impaired immune system regulation in the skin and gene expression changes characteristic of chronic liver damage.
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分子量
- 45 kDa
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基因ID
- 11839
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UniProt
- P31955
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途径
- RTK signaling, EGFR Signaling Pathway
抗原
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