ADA 抗体 (AA 135-363)
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北京 101111
Quick Overview for ADA 抗体 (AA 135-363) (ABIN6719272)
抗原
See all ADA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 135-363
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原理
- Anti-ADA Antibody (monoclonal, 6D4)
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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预测反应
- coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5%,82.9% amino acid (aa) sequence identity with mouse,rat ADA,respectively.
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产品特性
- Anti-ADA Antibody.(monoclonal, 6D4). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
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亚型
- IgG2b
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anti-Adenosine Deaminase (ADA) (AA 1-363) antibody
ADA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 287-314), (C-Term) antibodyADA 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31396 unconjugated
anti-Adenosine Deaminase (ADA) (AA 11-280) antibodyADA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G4-1C6 unconjugated
anti-Adenosine Deaminase (ADA) (AA 157-343) antibodyADA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 135-363) antibodyADA 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-241) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 65-170) antibodyADA 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x106 cells
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说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- ADA (Adenosine Deaminase (ADA))
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别名
- ADA
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背景
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Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Gene Full Name: adenosine deaminase
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分子量
- 45 kDa
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基因ID
- 100
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UniProt
- P00813
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
抗原
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