AES 抗体 (AA 69-138)
Quick Overview for AES 抗体 (AA 69-138) (ABIN6719264)
抗原
See all AES 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 69-138
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原理
- Anti-AES Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AES Antibody Picoband® (ABIN6719264). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human AES recombinant protein (Position: Y69-L138).
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亚型
- IgG
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anti-Amino-terminal Enhancer of Split (AES) (AA 1-264), (N-Term) antibody
AES 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (N-Term) antibodyVerified AES 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (C-Term) antibodyAES 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (C-Term), (N-Term) antibodyAES 适用: 人, 小鼠, 大鼠 WB, IF, EIA 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (AA 1-264) antibodyAES 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (AA 1-264) antibodyAES 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (C-Term) antibodyAES 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (N-Term) antibodyAES 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 非洲爪蟾, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (AA 143-192) antibodyAES 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 非洲爪蟾, 斑马鱼, 猴, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Amino-terminal Enhancer of Split (AES) (AA 1-264) antibodyAES 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartley, D. A., Preiss, A., Artavanis-Tsakonas, S. A deduced gene product from the Drosophila neurogenic locus, enhancer of split, shows homology to mammalian G-protein beta subunit. Cell 55: 785-795, 1988. 2. Hou, E. W., Li, S.-L. Genomic organization and chromosome localization to band 19p13.3 of the human AES gene: gene product exhibits strong similarity to the N-terminal domain of Drosophila enhancer of split Groucho protein. DNA Cell Biol. 17: 911-913, 1998. 3. Miyasaka, H., Choudhury, B. K., Hou, E. W., Li, S. S.-L.Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Europ. J. Biochem. 216: 343-352, 1993. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- AES (Amino-terminal Enhancer of Split (AES))
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别名
- AES
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背景
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Synonyms: Amino-terminal enhancer of split, Amino enhancer of split, Gp130-associated protein GAM, Grg-5, Groucho-related protein 5, Protein ESP1, Protein GRG, AES, GRG, GRG5
Tissue Specificity: Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Background: The AES gene is mapped to 19p13.3. The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
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分子量
- 22 kDa
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基因ID
- 166
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UniProt
- Q08117
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途径
- WNT signaling, SARS-CoV-2 Protein Interactome
抗原
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