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MFN1 抗体 (AA 651-741)

Name: MFN1 抗体 (AA 651-741)
SKU: ABIN670071
Availability: OutOfStock

This anti-MFN1 antibody is a 兔多克隆 antibody detecting MFN1 in WB, ELISA, FACS 和 IHC (p). Suitable for 小鼠, 大鼠, 兔和小鸡. This Primary Antibody has been cited in 3+ publications.

Rat brain lysates probed with Anti-Mfn1 Polyclonal Antibody at 1:5000 90min in 37˚C. (MFN1 抗体 (AA 651-741))

RSC96 probed with Mfn1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (MFN1 抗体 (AA 651-741))

FACS

Paraformaldehyde-fixed, paraffin embedded mouse testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. (MFN1 抗体 (AA 651-741))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. (MFN1 抗体 (AA 651-741))

IHC (p)

4 images

(3 references)

产品编号 ABIN670071

发货至: 中国

Datasheet as PDF

Quick Overview for MFN1 抗体 (AA 651-741) (ABIN670071)

抗原

See all MFN1 抗体

MFN1 (Mitofusin 1 (MFN1))

适用

小鼠, 大鼠, 兔, 小鸡

宿主

兔

克隆类型

多克隆

标记

This MFN1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product MFN1 Primary Antibody

抗原表位
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AA 651-741

交叉反应

小鸡, 小鼠, 兔, 大鼠

预测反应

Human,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Mfn 1

亚型

IgG
anti-Mitofusin 1 (MFN1) (AA 1-741) antibody
MFN1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3C9 unconjugated

anti-Mitofusin 1 (MFN1) (AA 1-234) antibody
MFN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 622-741) antibody
MFN1 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 622-741) antibody
MFN1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 745-756) antibody
MFN1 适用: 人, 兔, 犬, 马, 猴 IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (Internal Region) antibody
MFN1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 209-469) antibody
MFN1 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4D11 unconjugated

anti-Mitofusin 1 (MFN1) (AA 622-741) antibody
MFN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 1-227) antibody
MFN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 1-100) antibody
MFN1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Ku, Ji, Zhang, Li, Sang: "PM2.5, SO2 and NO2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain." in: Chemosphere, Vol. 163, pp. 27-34, (2016) (PubMed).

Qin, Deng, Lou, Yu, Pei, Guan et al.: "The decreased expression of mitofusin-1 and increased fission-1 together with alterations in mitochondrial morphology in the kidney of rats with chronic fluorosis may involve elevated oxidative ..." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), (2014) (PubMed).

Lou, Guan, Liu, Liu, Zhang, Pan, Pei: "The influence of chronic fluorosis on mitochondrial dynamics morphology and distribution in cortical neurons of the rat brain." in: Archives of toxicology, Vol. 87, Issue 3, pp. 449-57, (2013) (PubMed).
抗原

MFN1 (Mitofusin 1 (MFN1))

别名

Mfn1

背景

Synonyms: hfzo1, hfzo2, Mitofusin-1, Fzo homolog, Transmembrane GTPase MFN1, MFN1
Background: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.

基因ID

55669

UniProt

Q8IWA4