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KIT Ligand 抗体 (AA 151-250)

Name: KIT Ligand 抗体 (AA 151-250)
SKU: ABIN669936
Availability: OutOfStock

KITLG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Sp2/0 (Mouse myeloma cell) lysates probed with SCF Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (KIT Ligand 抗体 (AA 151-250))

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SCF Polyclonal Antibody (ABIN669936), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (KIT Ligand 抗体 (AA 151-250))

IHC

Formalin-fixed and paraffin embedded rat spleen tissue labeled with Anti-SCF Polyclonal Antibody (ABIN669936) , Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37 °C. (KIT Ligand 抗体 (AA 151-250))

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SCF Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (KIT Ligand 抗体 (AA 151-250))

IHC (p)

Mouse spleen cells probed with SCF Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange) (KIT Ligand 抗体 (AA 151-250))

5 images

(4 references)

产品编号 ABIN669936

发货至: 中国

Datasheet as PDF

抗原 See all KIT Ligand (KITLG) 抗体

KIT Ligand (KITLG)
抗原表位
15

7

7

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 151-250
适用
109

54

33

5

4

2

2

2

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
110

23

6

1
兔
Compatible Secondaries
克隆类型
109

31
多克隆
标记
70

15

7

6

5

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This KIT Ligand antibody is un-conjugated
应用范围
103

50

37

29

20

15

14

12

12

12

10

6

6

4

4

3

3

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human SCF

亚型

IgG
anti-KIT Ligand (KITLG) antibody
KITLG 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (AA 26-190) antibody
KITLG 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (AA 244-273) antibody
KITLG 适用: 人 WB, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (Middle Region) antibody
KITLG 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 山羊, 马, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (AA 180-229) antibody
KITLG 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 山羊, 马, 兔, 绵羊, 小鸡, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (Middle Region) antibody
KITLG 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) antibody
KITLG 适用: 人, 小鼠, 大鼠 WB, IHC (p), ELISA (Capture) 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (AA 201-234), (C-Term) antibody
KITLG 适用: 人 WB 宿主: 兔 Polyclonal RB50736 unconjugated

anti-KIT Ligand (KITLG) (AA 1-245) antibody
KITLG 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-KIT Ligand (KITLG) (AA 26-214) antibody
KITLG 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Yin, Liang, Wang, Yan, Yin, Wu, Su: "Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 649-658, (2018) (PubMed).

Lin, Tang, Cai, Wang, Li, Sui, Guo: "NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149031, (2016) (PubMed).

Wang, Wang, Li, Liu, Jiang, Li, Cao, Zhao, Wang, Luo: "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells." in: Tumour biology, Vol. 37, Issue 6, pp. 7213-27, (2016) (PubMed).

Goldstein, Goss, Hatzistergos, Rangel, Seidler, Saur, Hare: "Adult c-Kit(+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons." in: The Journal of comparative neurology, (2014) (PubMed).
抗原

KIT Ligand (KITLG)

别名

SCF (KITLG 产品)

背景

Synonyms: SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand, KITLG
Background: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

基因ID

4254

UniProt

P21583

途径

RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway