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Vimentin 抗体

VIM 适用: 人 WB, ELISA, IF, FM, MA 宿主: 小鸡 Polyclonal unconjugated
Rockland
产品编号 ABIN6699200
Supplier Product No.: 200-901-e54
发货至: 中国
  • 抗原 See all Vimentin (VIM) 抗体
    Vimentin (VIM)
    适用
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    宿主
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    小鸡
    克隆类型
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    多克隆
    标记
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    This Vimentin antibody is un-conjugated
    应用范围
    • 269
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    Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)
    Supplier Product No.
    200-901-e54
    Supplier
    Rockland
    原理
    Vimentin Antibody
    交叉反应 (详细)
    Anti-Vimentin antibody is directed against human Vimentin proteins.
    纯化方法
    The antibody was purified from chicken eggs as an IgY fraction.
    免疫原
    Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.
    亚型
    IgG
    Top Product
    Discover our top product VIM Primary Antibody
  • 应用备注

    ELISA_Dilution: 1:10,000

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:1000

    说明

    Anti-Vimentin antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appropriate cell lysate or extract.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Vimentin (VIM)
    别名
    Vimentin (VIM 产品)
    背景

    Synonyms: VIM

    Background: Vimentin antibody recognizes Vimentin which is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin antibody is ideal for investigators involved in Neuroscience, Cell Migration and Cancer Research.

    Gene Name: VIM

    基因ID
    7431
    UniProt
    P08670
    途径
    Caspase Cascade in Apoptosis
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