Vimentin 抗体
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- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
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适用
- 人
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This Vimentin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)
- Supplier Product No.
- 200-901-e54
- Supplier
- Rockland
- 原理
- Vimentin Antibody
- 交叉反应 (详细)
- Anti-Vimentin antibody is directed against human Vimentin proteins.
- 纯化方法
- The antibody was purified from chicken eggs as an IgY fraction.
- 免疫原
- Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.
- 亚型
- IgG
- Top Product
- Discover our top product VIM Primary Antibody
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- 应用备注
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ELISA_Dilution: 1:10,000
IF_Microscopy_Dilution: 1:500
Western_Blot_Dilution: 1:1000
- 说明
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Anti-Vimentin antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appropriate cell lysate or extract.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
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Synonyms: VIM
Background: Vimentin antibody recognizes Vimentin which is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin antibody is ideal for investigators involved in Neuroscience, Cell Migration and Cancer Research.
Gene Name: VIM
- 基因ID
- 7431
- UniProt
- P08670
- 途径
- Caspase Cascade in Apoptosis
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