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GAPDH 抗体 (AA 101-200)

Name: GAPDH 抗体 (AA 101-200)
SKU: ABIN668886
Availability: OutOfStock

GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

This image was generously provided by Loyda M. (GAPDH 抗体 (AA 101-200))

MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody at 1:300 overnight at 4˚C. (GAPDH 抗体 (AA 101-200))

Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody at 1:300 overnight at 4˚C. (GAPDH 抗体 (AA 101-200))

3 images

(4 references)

产品编号 ABIN668886

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
34

22

13

13

7

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-200
适用
211

145

128

64

44

39

25

25

23

19

19

18

15

15

14

13

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
158

86

16

9

4
兔
Compatible Secondaries
克隆类型
179

92
多克隆
标记
154

29

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
218

100

69

63

37

33

29

25

15

15

14

14

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

预测反应

Monkey

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse GAPDH

亚型

IgG

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Hu, Guo, Wang, Zhao, Zhang, Yang, Yu, Niu, Yang, Zheng, Wang: "Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors." in: Oncology letters, Vol. 11, Issue 2, pp. 1299-1304, (2016) (PubMed).

Zenón, Cantres-Rosario, Adiga, Gonzalez, Rodriguez-Franco, Langford, Melendez: "HIV-infected microglia mediate cathepsin B-induced neurotoxicity." in: Journal of neurovirology, (2015) (PubMed).

Wang, Ju, Sun, Hou, Yang, Zhang: "Overexpression of heparan sulfate 6-O-sulfotransferase-2 enhances fibroblast growth factor-mediated chondrocyte growth and differentiation." in: International journal of molecular medicine, Vol. 36, Issue 3, pp. 825-32, (2015) (PubMed).

Shou, Lin, Liang, Li, Chen: "Expression and prognosis of FOXO3a and HIF-1? in nasopharyngeal carcinoma." in: Journal of cancer research and clinical oncology, Vol. 138, Issue 4, pp. 585-93, (2012) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

背景

Synonyms: Gapd, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH
Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

基因ID

14433

UniProt

P16858