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CAMK2A 抗体

This anti-CAMK2A antibody is a 小鼠 单克隆 antibody detecting CAMK2A in WB, IHC, ELISA, IP, IF 和 FM. Suitable for 大鼠.
Rockland
产品编号 ABIN6658262
Supplier Product No.: 200-301-f01
发货至: 中国

Quick Overview for CAMK2A 抗体 (ABIN6658262)

抗原

See all CAMK2A 抗体
CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

适用

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大鼠

宿主

  • 73
  • 19
小鼠

克隆类型

  • 61
  • 31
单克隆

标记

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This CAMK2A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

克隆位点

22B1
  • Supplier Product No.

    200-301-f01

    Supplier

    Rockland

    原理

    CaMKII Antibody

    交叉反应 (详细)

    This monoclonal antibody is specific for CaMKII protein.

    纯化方法

    Anti-CaMKII Antibody was purified by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    CaMKII Antibody was produced in mice by repeated immunizations with synthetic peptide of rat CaMKII.

    亚型

    IgG1
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    ELISA_Dilution: 1:200

    IF_Microscopy_Dilution: User Optimized

    Western_Blot_Dilution: 1 μg/mL

    说明

    Anti-CaMKII Antibody is tested by IF, IP, WB, IHC, and ELISA. Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β). Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

    别名

    Camk2a

    背景

    Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha

    Background: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

    Gene Name: Camk2a

    基因ID

    25400

    NCBI登录号

    NP_037052

    UniProt

    P11275

    途径

    WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
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