CaMKII alpha/beta (pThr286) 抗体
Quick Overview for CaMKII alpha/beta (pThr286) 抗体 (ABIN6658207)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr286
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Supplier Product No.
- 612-401-c71
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Supplier
- Rockland
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原理
- CaM Kinase II phospho T286 Antibody
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交叉反应 （详细）
- Anti-CaM Kinase II pT286 Antibody is directed against CaM Kinase II protein phosphorylated at T286.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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免疫原
- Anti-CaM Kinase II pT286 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286.
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亚型
- IgG
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anti-CaMKII alpha/beta (Internal Region) antibody
Verified 适用: 大鼠, 人, 小鼠 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-CaMKII alpha/beta (pThr286) antibody适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-CaMKII alpha/beta (pThr306) antibody适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
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说明
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Anti-CaM Kinase II pT286 antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to alpha CaM Kinase II and 60 kDa corresponding to beta CaM Kinase II proteins phosphorylated at Thr286.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- CaMKII alpha/beta
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别名
- Camk2A/B
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背景
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Synonyms: Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Camk2a protein
Background: CaM Kinase II antibody reacts with Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) which is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. Anti-CaM Kinase II antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Gene Name: CAMK2A/B
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基因ID
- 25400
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NCBI登录号
- NP_037052
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UniProt
- P11275
抗原
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