CaMKII alpha/beta (Internal Region) 抗体
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- 抗原
- CaMKII alpha/beta
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抗原表位
- Internal Region
- 适用
- 大鼠, 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- CAMK2
- 序列
- PRTAQSEETR VWHR
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
- 亚型
- IgG
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anti-CaMKII alpha/beta (pThr286) antibody
适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-CaMKII alpha/beta (pThr306) antibody适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: Approx 48-50+60 kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60 kDa in Human Cerebellum lysates (calculated MW of 55.3 kDa according to Human NP
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10u
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- CaMKII alpha/beta
- 别名
- CAMK2A/B
- 背景
- CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase
- 基因ID
- 815, 816, 12322
- NCBI登录号
- NP_057065, NP_741960
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