CAMK2A 抗体
Quick Overview for CAMK2A 抗体 (ABIN6658151)
抗原
See all CAMK2A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f00
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Supplier
- Rockland
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原理
- CaMKII Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with CaMKII from mouse, rat, and bovine based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-CaMKII Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- CaMKII Antibody was produced in mice by repeated immunizations with a partially purified rat CaMKII.
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亚型
- IgG1
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anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibody
CAMK2A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C4 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyMicroarray verified CAMK2A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB443776 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibodyCAMK2A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibodyCAMK2A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibodyCAMK2A 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 1:200
Immunohistochemistry_Dilution: 1:2000
IF_Microscopy_Dilution: 1:50
Western_Blot_Dilution: 0.1 μg/mL (1:10,000)
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说明
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Anti-CaMKII Antibody has been tested in IF, IP, WB, IHC and RIA/ELISA. Antibody is provided in PBS pH 7.4. Expect a band approximately ~50-60kDa protein, corresponding to the molecular mass of CaMKII α-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: See application note.
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
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别名
- Camk2a
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背景
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Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha
Background: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.
Gene Name: Camk2a
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基因ID
- 25400
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NCBI登录号
- NP_037052
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UniProt
- P11275
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途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
抗原
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