MECP2 抗体 (pSer421)
(1 reference)
Quick Overview for MECP2 抗体 (pSer421) (ABIN6658009)
抗原
See all MECP2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer421
-
Supplier Product No.
- 600-401-x14
-
Supplier
- Rockland
-
原理
- MeCP2 Phospho S421 Antibody
-
交叉反应 （详细）
- Anti-Phospho-Ser421 MeCP2 Antibody is directed against MeCP2 protein phosphorylated at S421.
-
纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
-
过滤
- Sterile filtered
-
免疫原
- Anti-Phospho-Ser421 MeCP2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2 protein conjugated to KLH.
-
亚型
- IgG
-
-
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 7-148) antibody
MECP2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8H4A5B9 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-486) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (pSer423) antibodyMECP2 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB29261 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (C-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 400-428) antibodyMECP2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB25959 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4B6 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (N-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
-
说明
-
Anti-Phospho-Ser421 MeCP2 Antibody is tested for Western Blots and is specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
-
有效期
- 12 months
-
-
-
: "The Osteogenic Niche Is a Calcium Reservoir of Bone Micrometastases and Confers Unexpected Therapeutic Vulnerability." in: Cancer cell, Vol. 34, Issue 5, pp. 823-839.e7, (2019) (PubMed).
-
: "The Osteogenic Niche Is a Calcium Reservoir of Bone Micrometastases and Confers Unexpected Therapeutic Vulnerability." in: Cancer cell, Vol. 34, Issue 5, pp. 823-839.e7, (2019) (PubMed).
-
- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
-
别名
- MeCP2
-
背景
-
Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein
Background: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MeCP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment. MeCP2 pS421 Antibody is ideal for researchers interested in neuroscience research.
Gene Name: MECP2
-
基因ID
- 17257
-
NCBI登录号
- NP_001075448
-
UniProt
- Q9Z2D6
-
途径
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
抗原
-
