SIX3 抗体 (Internal Region)
(6 references)
Quick Overview for SIX3 抗体 (Internal Region) (ABIN6657913)
抗原
See all SIX3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-a26s
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Supplier
- Rockland
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原理
- Six3 Antibody
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交叉反应 （详细）
- This antibody reacts with mouse Six3.
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
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亚型
- IgG
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anti-Sine Oculis-Related Homeobox 3 (SIX3) (AA 273-332) antibody
SIX3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (Internal Region) antibodySIX3 适用: 小鼠 WB, ELISA, IHC, IF, FM, DB 宿主: 豚鼠 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (AA 273-332) antibodySIX3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D12 unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (Internal Region) antibodySIX3 适用: 小鼠 WB, ELISA, IHC, IF, FM, DB 宿主: 豚鼠 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (C-Term) antibodySIX3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (Internal Region) antibodySIX3 适用: 小鼠 WB, ELISA, IHC, FM 宿主: 兔 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (AA 1-332) antibodySIX3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (C-Term) antibodySIX3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Sine Oculis-Related Homeobox 3 (SIX3) (N-Term) antibodySIX3 适用: 人, 小鼠, 大鼠, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000-1:15,000
Immunohistochemistry_Dilution: 1:100-1:250
IF_Microscopy_Dilution: 1:200 - 1:1,000
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized
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说明
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Suggested Applications: IF, Multiplex
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 3 months
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: "Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development." in: Neuron, Vol. 107, Issue 4, pp. 684-702.e9, (2020) (PubMed).
: "PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice." in: eLife, Vol. 8, (2020) (PubMed).
: "Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations." in: Frontiers in molecular neuroscience, Vol. 12, pp. 212, (2019) (PubMed).
: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." in: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).
: "Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).
: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." in: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).
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: "Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development." in: Neuron, Vol. 107, Issue 4, pp. 684-702.e9, (2020) (PubMed).
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- SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
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别名
- Six3
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背景
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Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3
Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain. Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product. Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.
Gene Name: Six3
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基因ID
- 20473, 59939908
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UniProt
- Q62233
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途径
- Protein targeting to Nucleus
抗原
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