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SIX3 抗体 (Internal Region)

SIX3 适用: 小鼠 WB, ELISA, IHC, FM 宿主: 豚鼠 Polyclonal unconjugated
产品编号 ABIN6657910
发货至: 中国
  • 抗原 See all SIX3 抗体
    SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
    抗原表位
    • 15
    • 8
    • 8
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 49
    • 18
    • 16
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    小鼠
    宿主
    • 49
    • 3
    • 1
    豚鼠
    克隆类型
    • 51
    • 2
    多克隆
    标记
    • 22
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIX3 antibody is un-conjugated
    应用范围
    • 45
    • 21
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    纯化方法
    This product was purified by Protein A chromatography from monospecific antiserum.  This antibody reacts with mouse Six3.   Cross-reactivity with Six3 from other sources has not been determined.
    免疫原

    Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

    Immunogen Type: Peptide

    亚型
    IgG
    Top Product
    Discover our top product SIX3 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:250 - 1:500

    Application Note: This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

    ELISA Dilution: 1:5,000 - 1:25,000

    Western Blot Dilution: 1:500 - 1:2,000

    IF Microscopy Dilution: 1:250 - 1:500

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Madrigal, Moreno-Bravo, Martínez-López, Martínez, Puelles: "Mesencephalic origin of the rostral Substantia nigra pars reticulata." in: Brain structure & function, Vol. 221, Issue 3, pp. 1403-12, (2016) (PubMed).

    Moldrich, Gobius, Pollak, Zhang, Ren, Brown, Mori, De Juan Romero, Britanova, Tarabykin, Richards: "Molecular regulation of the developing commissural plate." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3645-61, (2010) (PubMed).

  • 抗原
    SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
    别名
    SIX3 (SIX3 产品)
    背景

    Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

    Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

    Gene Name: SIX3

    基因ID
    20473, 59939908
    UniProt
    Q62233
    途径
    Protein targeting to Nucleus
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