CD11b 抗体 (PE)
Quick Overview for CD11b 抗体 (PE) (ABIN6657853)
抗原
See all CD11b (ITGAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-508-n79
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Supplier
- Rockland
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原理
- CD11b Phycoerythrin Antibody
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交叉反应 （详细）
- Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine).
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纯化方法
- Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b.
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过滤
- Sterile filtered
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免疫原
- Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.
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亚型
- IgG2b kappa
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Labeling Ratio
- 1-2
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anti-Integrin alpha M (ITGAM) (AA 520-570) antibody
ITGAM 适用: 人, 小鼠, 大鼠 FACS 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 145-340) antibodyITGAM 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 77-180) antibodyITGAM 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 253-282), (N-Term) antibodyITGAM 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB17991 unconjugated
anti-Integrin alpha M (ITGAM) (AA 31-160) antibodyITGAM 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3338 unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3340 unconjugated
anti-Integrin alpha M (ITGAM) antibody (FITC)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 FITC
anti-Integrin alpha M (ITGAM) antibody (Biotin)ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 Biotin
anti-Integrin alpha M (ITGAM) antibody (PE)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
IF_Microscopy_Dilution: 0.25 μg/10x6 cells
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说明
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Anti-CD11b is tested for FLOW and IF, and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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别名
- CD11b
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背景
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Synonyms: CD11b
Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Gene Name: Itgam
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UniProt
- A1E2I0
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途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
抗原
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