STAT4 抗体 (AA 730-749)
Quick Overview for STAT4 抗体 (AA 730-749) (ABIN6657793)
抗原
See all STAT4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 730-749
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Supplier Product No.
- 100-401-861
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Supplier
- Rockland
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原理
- Stat4 Antibody
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交叉反应 （详细）
- Reactivity occurs against mouse STAT4 protein.
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纯化方法
- This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4.
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亚型
- IgG
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anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 505-737) antibody
STAT4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 1-748) antibodySTAT4 适用: 人 WB, ELISA, IP, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C2-1C12 unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 600-700) antibodySTAT4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 569-748) antibodySTAT4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (pTyr693) antibodySTAT4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) antibodySTAT4 适用: 人 WB, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (N-Term) antibodySTAT4 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (C-Term) antibodySTAT4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (pTyr693) antibodySTAT4 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 1-749) antibodySTAT4 适用: 人 WB, IHC, ELISA, IP, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C2-1C12 unconjugated
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应用备注
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Immunoprecipitation_Dilution: Use 5 μL
ELISA_Dilution: 1:5,000 - 1:25,000
Western_Blot_Dilution: 1:5,000
Other: User Optimized
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说明
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This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for immunoprecipitation. A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. Reactivity in other immunoassays is unknown.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))
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别名
- STAT4
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背景
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Synonyms: rabbit anti-STAT4 Antibody, Signal transducer and activator of transcription 4, Stat4
Background: Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription. STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members. STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.
Gene Name: STAT4
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基因ID
- 20849
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UniProt
- P42228
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途径
- JAK/STAT Signaling
抗原
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