HSF1 抗体 (AA 425-439) (HRP)
Quick Overview for HSF1 抗体 (AA 425-439) (HRP) (ABIN6657642)
抗原
See all HSF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 425-439
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Supplier Product No.
- 200-503-w89
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Supplier
- Rockland
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原理
- HSF1 Antibody Peroxidase
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纯化方法
- Anti-HSF1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439.
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亚型
- IgG1
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390
anti-Heat Shock Factor Protein 1 (HSF1) (AA 350-529) antibodyHSF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 1:10,000
Gel_Shift_Dilution: User Optimized
Western_Blot_Dilution: 1:1000
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说明
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Anti-HSF1 HRP Conjugated Antibody is suitable for Western Blots, Immunoprecipitation, ELISA, Gel Mobility Shift Assay and Immunocytochemistry. Expect a band approximately ~85kDa protein in unstressed cell lysates, and a 95 kDa protein in heat shocked cell lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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别名
- HSF1
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背景
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Synonyms: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1
Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). Anti-HSF1 Antibody is ideal for research in Genetics, Transcription, Cell Signaling and pathways including ERK and MAPK.
Gene Name: Hsf1
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基因ID
- 15499
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NCBI登录号
- NP_058761
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UniProt
- P38532
抗原
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