Hemagglutinin 抗体
(4 references)
Quick Overview for Hemagglutinin 抗体 (ABIN6657576)
抗原
See all Hemagglutinin (HA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Virus Strain
- A/Vietnam/1203/04
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Supplier Product No.
- 500-301-976
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Supplier
- Rockland
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原理
- H5N1 Antibody VN04-8
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交叉反应 （详细）
- VN04-8 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes.
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纯化方法
- This product was clarified from mouse ascitic fluid and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].
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过滤
- Sterile filtered
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免疫原
- This monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 mug HA with incomplete Freund's adjuvant, administered 3 week apart.
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亚型
- IgG1 kappa
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anti-Hemagglutinin antibody (Influenza A Virus) (AA 128-143)
HA 适用: Influenza A Virus WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hemagglutinin antibody (Influenza A Virus H9N2) (AA 9-28)HA 适用: Influenza A Virus H9N2 WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hemagglutinin antibody (Influenza A Virus H1N1 (A/California/04/2009)) (AA 151-250)HA 适用: Influenza A Virus H1N1, Virus ELISA, WB, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Hemagglutinin antibody (Influenza A Virus) (AA 17-338)HA 适用: Influenza A Virus WB 宿主: 小鼠 Monoclonal 4E10C10 unconjugated
anti-Hemagglutinin antibody (Influenza Virus)HA 适用: Influenza Virus ELISA, IP, WB, DB 宿主: 小鼠 Monoclonal HA-C5 unconjugated
anti-Hemagglutinin antibody (Influenza A Virus H3N2 (A/Shangdong/9/93))HA 适用: Influenza A Virus H3N2 ELISA, WB 宿主: 小鼠 Monoclonal B264M unconjugated
anti-Hemagglutinin antibody (Influenza A Virus) (AA 72-88)HA 适用: Influenza A Virus WB 宿主: 兔 Polyclonal unconjugated
anti-Hemagglutinin antibody (Influenza A Virus H1N1 (A/Swine/Iowa/15/1930 H1N1)) (AA 205-220)HA 适用: Influenza A Virus H1N1 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization_Dilution: User Optimized
Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 1:5,000
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: User Optimized
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说明
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This monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).
: "Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge." in: The Journal of infectious diseases, Vol. 194, Issue 2, pp. 159-67, (2006) (PubMed).
: "Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice." in: Respiratory research, Vol. 7, pp. 126, (2006) (PubMed).
: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, (2005) (PubMed).
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: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).
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- Hemagglutinin (HA)
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别名
- Hemagglutinin
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物质类
- Influenza Protein
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背景
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Synonyms: mouse anti-H5N1 antibody, mouse anti-Hemagglutinin A antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody
Background: Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza. This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.
Gene Name: HA
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基因ID
- 159144921
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UniProt
- A8UDQ2
抗原
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