Lamin B1 抗体 (C-Term)
Quick Overview for Lamin B1 抗体 (C-Term) (ABIN6657537)
抗原
See all Lamin B1 (LMNB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-p62
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Supplier
- Rockland
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原理
- Lamin B1 Antibody
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交叉反应 (详细)
- Anti-Lamin B1 antibody is directed against human Lamin B1 protein.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- Lamin B1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B1.
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亚型
- IgG
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应用备注
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Immunohistochemistry_Dilution: 0.5-1 μg/mL
Western_Blot_Dilution: 0.1-0.5 μg/mL
Other: Immunocytochemistry: 0.5-1 μg/mL
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说明
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Anti-Lamin B1 is tested for Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~66.4 kDa corresponding to the appropriate cell lysate or extract. WB tested in Human, Mouse, Rat, IHC-P (heat) and IHC-F in human and rat, ICC in human, and ICC/IF in human.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide
Stabilizer: 5 mg BSA
Preservative: 0.05 mg Thimerosal -
储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A requirement for Krüppel Like Factor-4 in the maintenance of endothelial cell quiescence." in: Frontiers in cell and developmental biology, Vol. 10, pp. 1003028, (2022) (PubMed).
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: "A requirement for Krüppel Like Factor-4 in the maintenance of endothelial cell quiescence." in: Frontiers in cell and developmental biology, Vol. 10, pp. 1003028, (2022) (PubMed).
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- Lamin B1 (LMNB1)
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别名
- Lamin B1
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背景
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Synonyms: LMNB1, LMN2, LMNB, Lamin B1 antibody
Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This antibody is suitable for researchers interested in chromatin antibody research.
Gene Name: LMNB1
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基因ID
- 4001
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NCBI登录号
- NP_001172028.11
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UniProt
- P20700
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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