Involucrin 抗体
Quick Overview for Involucrin 抗体 (ABIN6657536)
抗原
See all Involucrin (IVL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p39
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Supplier
- Rockland
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原理
- Involucrin Antibody
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交叉反应 (详细)
- This product reacts with human and pig.
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纯化方法
- Anti-Involucrin antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Involucrin antibody was produced in mice by repeated immunizations with human involucrin.
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亚型
- IgG1
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应用备注
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ELISA_Dilution: 1:20,000
Immunohistochemistry_Dilution: 1:100-1:500
Western_Blot_Dilution: 1 μg/mL
Other: Immunocytochemistry: 1 μg/mL
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说明
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Anti-Involucrin is tested for Immunohistochemistry -P, -F, Immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~68.5 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Involucrin (IVL)
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别名
- Involucrin
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背景
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Synonyms: INVO, IVL, Involucrin Antibody
Background: Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated. This antibody is suitable for researchers interested in skin cell research.
Gene Name: IVL
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基因ID
- 3713
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NCBI登录号
- NP_005538
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UniProt
- P07476
抗原
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