CD34 抗体 (C-Term)
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北京 101111
Quick Overview for CD34 抗体 (C-Term) (ABIN6657527)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-p90
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Supplier
- Rockland
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原理
- CD34 Antibody
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交叉反应 (详细)
- Anti-CD34 antibody is directed against human CD34 protein.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- CD34 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human CD34.
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亚型
- IgG
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应用备注
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Immunohistochemistry_Dilution: 0.5-1 μg/mL
Flow_Cytometry_Dilution: 1-3 μg/1x106 cells
Western_Blot_Dilution: 0.1-0.5 μg/mL
Other: Immunocytochemistry: 0.5-1 μg/mL
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说明
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Anti-CD34 is tested for Flow Cytometry, Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~40.7 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide
Stabilizer: 5 mg BSA
Preservative: 0.05 mg Thimerosal -
储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CD34
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别名
- CD34
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背景
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Synonyms: Hematopoietic progenitor cell antigen CD34, CD34 antibody
Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis. This antibody is suitable for researchers interested in stem cell, cancer and neuroscience research.
Gene Name: CD34
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基因ID
- 947
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NCBI登录号
- NP_001020280
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UniProt
- P28906
抗原
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