8-OHDG 抗体
(6 references)
Quick Overview for 8-OHDG 抗体 (ABIN6657492)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-a99
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Supplier
- Rockland
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原理
- 8-Hydroxy Guanine Antibody
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纯化方法
- This Protein G purified Anti-8-Hydroxy Guanine monoclonal antibody recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
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过滤
- Sterile filtered
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免疫原
- This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 8-hydroxy-guanosine-BSA and casein conjugates.
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亚型
- IgG2b
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anti-8-Hydroxyguanosine (8-OHDG) antibody
8-OHDG ELISA, IHC 宿主: 小鼠 Monoclonal N45-1 unconjugated
anti-8-Hydroxyguanosine (8-OHDG) antibody8-OHDG ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-8-Hydroxyguanosine (8-OHDG) antibody8-OHDG IHC (p), EIA, IHC (fro) 宿主: 小鼠 Monoclonal 15A3 unconjugated
anti-8-Hydroxyguanosine (8-OHDG) antibody8-OHDG ELISA, IHC, WB 宿主: 小鼠 Monoclonal 15A3 unconjugated
anti-8-Hydroxyguanosine (8-OHDG) antibody8-OHDG IHC (p), EIA, IF 宿主: 小鼠 Monoclonal N45-1 unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: User Optimized
Immunohistochemistry_Dilution: 1:1000
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: User Optimized
Other: User Optimized
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说明
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Suggested Applications: IF, IP, WB
This Protein G purified antibody has been tested for use in immunohistochemistry, ICC/IF, Dot Blot, IP, Flow Cytometry, and ELISA. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-8-Hydroxy Guanine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Alzheimer's Amyloid-β Accelerates Cell Senescence and Suppresses SIRT1 in Human Neural Stem Cells." in: Biomolecules, Vol. 14, Issue 2, (2024) (PubMed).
: "Is microglial dystrophy a form of cellular senescence? An analysis of senescence markers in the aged human brain." in: Glia, Vol. 71, Issue 2, pp. 377-390, (2022) (PubMed).
: "Idebenone attenuates cerebral inflammatory injury in ischemia and reperfusion via dampening NLRP3 inflammasome activity." in: Molecular immunology, Vol. 123, pp. 74-87, (2020) (PubMed).
: "New mitochondrial DNA synthesis enables NLRP3 inflammasome activation." in: Nature, Vol. 560, Issue 7717, pp. 198-203, (2019) (PubMed).
: "Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy." in: Autophagy, Vol. 11, Issue 1, pp. 166-82, (2015) (PubMed).
: "Oxidized mitochondrial DNA activates the NLRP3 inflammasome during apoptosis." in: Immunity, Vol. 36, Issue 3, pp. 401-14, (2012) (PubMed).
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: "Alzheimer's Amyloid-β Accelerates Cell Senescence and Suppresses SIRT1 in Human Neural Stem Cells." in: Biomolecules, Vol. 14, Issue 2, (2024) (PubMed).
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- 8-OHDG (8-Hydroxyguanosine (8-OHDG))
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别名
- 8-Hydroxy Guanine
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物质类
- Chemical
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背景
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Synonyms: 8 hydroxy 2' deoxyguanosine antibody, 8 hydroxyguanine antibody, 8 hydroxyguanosine antibody, 8 OHG antibody, 8-OHG antibody, 8OG antibody, 8OHdG antibody, 8OHG antibody, 8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, DNA/RNA Damage Antibody
Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA damage due to endogenous cellular processes: oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS may develop. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS cause a variety of other issues in addition to DNA damage. 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring. Finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has been tested specifically in the development of diabetes, hypertension and strokes.
抗原
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