Dystrophin 抗体
Quick Overview for Dystrophin 抗体 (ABIN6657490)
抗原
See all Dystrophin (DMD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p36
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Supplier
- Rockland
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原理
- Dystrophin Antibody
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交叉反应 （详细）
- This product reacts with human, mouse, rat, and rabbit.
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纯化方法
- Anti-Dystrophin antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Dystrophin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to human dystrophin fragment.
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亚型
- IgG2b
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anti-Dystrophin (DMD) (AA 346-635) antibody
DMD 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) antibodyDMD 适用: 人, 小鼠, 大鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 1-635) antibodyDMD 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3242 unconjugated
anti-Dystrophin (DMD) (AA 3076-3404) antibodyDMD 适用: 人, 小鼠, 大鼠 IHC, WB, ICC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 3048-3328) antibodyDMD 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3241 unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3245 unconjugated
anti-Dystrophin (DMD) (AA 253-597) antibodyDMD 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 3400-3558) antibodyDMD 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:20,000
Immunohistochemistry_Dilution: 1:100-1:500
Western_Blot_Dilution: 1-2 μg/mL
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说明
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Anti-Dystrophin is tested for Immunohistochemistry -F and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~425 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 200μL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Dystrophin (DMD)
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别名
- Dystrophin
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背景
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Synonyms: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
Background: Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb (2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons, the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects. This antibody is suitable for researchers interested in Muscular Dystrophies and Dystrophin-Glycoprotein Complexes and cardiovascular research.
Gene Name: DMD
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基因ID
- 1756
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NCBI登录号
- NP_000100
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UniProt
- P11532
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途径
- Skeletal Muscle Fiber Development
抗原
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