Desmin 抗体
Quick Overview for Desmin 抗体 (ABIN6657489)
抗原
See all Desmin (DES) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p35
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Supplier
- Rockland
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原理
- Desmin Antibody
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交叉反应 (详细)
- This product reacts with human, mouse, rat, and rabbit.
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纯化方法
- Anti-Desmin antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Desmin antibody was produced in mice by repeated immunizations with desmin from pig stomach.
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亚型
- IgG1
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应用备注
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ELISA_Dilution: 1:20,000
Immunohistochemistry_Dilution: 1:100-1:500
Western_Blot_Dilution: 2 μg/mL
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说明
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Anti-Desmin is tested for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~53.5 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Desmin (DES)
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别名
- Desmin
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背景
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Synonyms: CMD1I, CSM1, CSM2, intermediate filament protein
Background: Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, neurodegenerative diseases, cell adhesion research, and cytoskeletal signaling research.
Gene Name: DES
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基因ID
- 396725
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NCBI登录号
- NP_001001535
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UniProt
- P02540
抗原
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