Paxillin 抗体 (AA 305-559)
Quick Overview for Paxillin 抗体 (AA 305-559) (ABIN6657483)
抗原
See all Paxillin (PXN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 305-559
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Supplier Product No.
- 200-301-p44
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Supplier
- Rockland
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原理
- Paxillin Antibody
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交叉反应 （详细）
- This product reacts with human, mouse, rat, and chicken.
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纯化方法
- Anti-Paxillin antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Paxillin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to the C-terminal part of chicken paxillin (amino acids 305-559).
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亚型
- IgG1
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anti-Paxillin (PXN) (AA 201-461) antibody
PXN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 347-578) antibodyPXN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 1-262) antibodyPXN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 327-568) antibodyPXN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 456-472) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 456-472), (C-Term) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (pTyr118) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 1-262) antibodyPXN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (pSer178) antibodyPXN 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 28-45) antibodyPXN 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:20,000
Western_Blot_Dilution: 1-2 μg/mL
Other: ICC: 1:100-1:500
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说明
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Anti-Paxillin is useful for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~64.5 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Paxillin (PXN)
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别名
- Paxillin
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背景
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Synonyms: Paxillin antibody, PXN
Background: The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68- kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. This antibody is suitable for researchers interested in growth factors and signal trasduction.
Gene Name: PXN
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基因ID
- 5829
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NCBI登录号
- NP_001074324
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UniProt
- P49023
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途径
- MAPK Pathway, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
抗原
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