TLR9 抗体
Quick Overview for TLR9 抗体 (ABIN6656659)
抗原
See all TLR9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-i31
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Supplier
- Rockland
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原理
- TLR9 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with Anti-TLR9 from monkey based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-TLR9 Antibody was purified by Protein G chromatography.
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免疫原
- TLR9 Antibody was produced in mice prepared repeated immunizations with amino acids corresponding to an internal sequence of human TLR9 isoform A protein.
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亚型
- IgG1 kappa
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应用备注
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Immunohistochemistry_Dilution: 10-20 μg/mL (frozen)
Flow_Cytometry_Dilution: 0.1-2 μg/10^6 cells
Western_Blot_Dilution: 1-5 μg/mL
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说明
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Anti-TLR9 Antibody is tested for use in WB, DB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 113kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- TLR9 (Toll-Like Receptor 9 (TLR9))
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别名
- TLR9
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背景
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Synonyms: Toll-like receptor 9
Background: Anti-TLR9 CD289 Antibody detects human TLR9 CD289. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, a cDNA coding for TLR9 has been identified and subsequently isolated. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5 % . TLR9 is highly expressed in spleen. Anti-TLR9 CD289 Antibody is ideal for investigators involved in cytokines and growth factor research.
Gene Name: TLR9
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基因ID
- 54106
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NCBI登录号
- NP_059138
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UniProt
- Q9NR96
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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