MAP1LC3A 抗体 (N-Term)
(1 reference)
Quick Overview for MAP1LC3A 抗体 (N-Term) (ABIN6656617)
抗原
See all MAP1LC3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 200-401-h57
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Supplier
- Rockland
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原理
- ATG8 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-ATG8 Antibody was purified by Protein G chromatography.
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免疫原
- ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.
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亚型
- IgG
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anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (cleaved) antibody
MAP1LC3A 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal RB38908 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibodyMAP1LC3A 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5B10D8 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibodyMAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7481 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-120) antibodyMAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibodyMAP1LC3A 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibodyMAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 166AT1234 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibodyMAP1LC3A 适用: 人 WB, DB, EIA 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 30-56) antibodyMAP1LC3A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11840 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibodyMAP1LC3A 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibodyMAP1LC3A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7E9E12 unconjugated
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应用备注
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Immunohistochemistry_Dilution: 5-10 μg/mL
Western_Blot_Dilution: 0.25-1 μg/mL
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说明
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Suggested Applications: IF
Anti-ATG8 antibody is tested by IHC-P and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course." in: Brain : a journal of neurology, Vol. 142, Issue 12, pp. 3876-3891, (2020) (PubMed).
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: "Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course." in: Brain : a journal of neurology, Vol. 142, Issue 12, pp. 3876-3891, (2020) (PubMed).
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- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
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别名
- MAP1LC3A
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背景
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Synonyms: Activating transcription factor 8
Background: Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.
Gene Name: MAP1LC3A
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基因ID
- 84557
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NCBI登录号
- NP_115903
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UniProt
- Q9H492
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途径
- Autophagy
抗原
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